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Robert steinhoff eth Analytical chemistry 85 20 , , Juan Zhang, Minisymposium with the group of Prof. Niels Scheuschner, Dr. Single Cell Metabolism: Methods and Protocols, , Analyst 22 , ,
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This approach allowed a comprehensive insight on metabolic as well as protein expression level. Identified features were used to explain differences between the. Monitoring intracellular component pools to identify steady state in mammalian cell perfusion culture � Daniel Karst � Robert Steinhoff � Marie Kopp � Renato Zenobi. Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures.
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Perfusion cultures of mammalian cells are a well-considered alternative in the process development for new biologic products. The system can't perform the operation now. Although macroscopic variables such as viable cell density, main metabolite or product concentrations are observed constant, little is known about the extent of steady state on intracellular level. This approach allowed a comprehensive insight on metabolic as well as protein expression level. International Journal of Mass Spectrometry , ,